es 2 Search Results


es 2  (ATCC)
97
ATCC es 2
Es 2, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human ovarian cancer cell lines  (ATCC)
94
ATCC human ovarian cancer cell lines
Human Ovarian Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell lysates  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology cell lysates
Cell Lysates, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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endosidin2  (MedChemExpress)
94
MedChemExpress endosidin2
Endosidin2, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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endosidin 2 t37020  (TargetMol)
92
TargetMol endosidin 2 t37020
Endosidin 2 T37020, supplied by TargetMol, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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es2  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection es2
Figure 1. Inhibition of EZH2 decreases BRCA1 mRNA level but increases BRCA1 protein level. (A) The mRNA level of BRCA1 was decreased in shEZH2 transfected cells compared with untransfected <t>ES2,</t> SKOV3, and A2780 cells. (B) Immunoblot assays revealed that total protein expression of BRCA1 was increased in stable transfected shEZH2-A2780 cells. (C) Left, loss of EZH2 increased BRCA1 nuclear protein level, but decreased BRCA1 cytoplasmic protein level. N and C represent nuclear and cytoplasmic protein respectively. Right, quantization of western blot. (D) Immunocytochemistry analysis shown that stable inhibition of EZH2 in A2780 cells increased BRCA1 protein expression and induced BRCA1 nuclear localization (×400).
Es2, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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heya b es-2  (Bioscientifica Ltd)
90
Bioscientifica Ltd heya b es-2
Figure 1. Inhibition of EZH2 decreases BRCA1 mRNA level but increases BRCA1 protein level. (A) The mRNA level of BRCA1 was decreased in shEZH2 transfected cells compared with untransfected <t>ES2,</t> SKOV3, and A2780 cells. (B) Immunoblot assays revealed that total protein expression of BRCA1 was increased in stable transfected shEZH2-A2780 cells. (C) Left, loss of EZH2 increased BRCA1 nuclear protein level, but decreased BRCA1 cytoplasmic protein level. N and C represent nuclear and cytoplasmic protein respectively. Right, quantization of western blot. (D) Immunocytochemistry analysis shown that stable inhibition of EZH2 in A2780 cells increased BRCA1 protein expression and induced BRCA1 nuclear localization (×400).
Heya B Es 2, supplied by Bioscientifica Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdna prepared from rna extracted from ovary cell line es2  (BioChain Institute)
90
BioChain Institute cdna prepared from rna extracted from ovary cell line es2
Figure 1. Inhibition of EZH2 decreases BRCA1 mRNA level but increases BRCA1 protein level. (A) The mRNA level of BRCA1 was decreased in shEZH2 transfected cells compared with untransfected <t>ES2,</t> SKOV3, and A2780 cells. (B) Immunoblot assays revealed that total protein expression of BRCA1 was increased in stable transfected shEZH2-A2780 cells. (C) Left, loss of EZH2 increased BRCA1 nuclear protein level, but decreased BRCA1 cytoplasmic protein level. N and C represent nuclear and cytoplasmic protein respectively. Right, quantization of western blot. (D) Immunocytochemistry analysis shown that stable inhibition of EZH2 in A2780 cells increased BRCA1 protein expression and induced BRCA1 nuclear localization (×400).
Cdna Prepared From Rna Extracted From Ovary Cell Line Es2, supplied by BioChain Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ultrasonic irradiation apparatus ultrasonic apparatus es-2  (Og Giken Co Ltd)
90
Og Giken Co Ltd ultrasonic irradiation apparatus ultrasonic apparatus es-2
Figure 1. Inhibition of EZH2 decreases BRCA1 mRNA level but increases BRCA1 protein level. (A) The mRNA level of BRCA1 was decreased in shEZH2 transfected cells compared with untransfected <t>ES2,</t> SKOV3, and A2780 cells. (B) Immunoblot assays revealed that total protein expression of BRCA1 was increased in stable transfected shEZH2-A2780 cells. (C) Left, loss of EZH2 increased BRCA1 nuclear protein level, but decreased BRCA1 cytoplasmic protein level. N and C represent nuclear and cytoplasmic protein respectively. Right, quantization of western blot. (D) Immunocytochemistry analysis shown that stable inhibition of EZH2 in A2780 cells increased BRCA1 protein expression and induced BRCA1 nuclear localization (×400).
Ultrasonic Irradiation Apparatus Ultrasonic Apparatus Es 2, supplied by Og Giken Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sodium laureth sulfate standapol® es-2  (BASF)
90
BASF sodium laureth sulfate standapol® es-2
Figure 1. Inhibition of EZH2 decreases BRCA1 mRNA level but increases BRCA1 protein level. (A) The mRNA level of BRCA1 was decreased in shEZH2 transfected cells compared with untransfected <t>ES2,</t> SKOV3, and A2780 cells. (B) Immunoblot assays revealed that total protein expression of BRCA1 was increased in stable transfected shEZH2-A2780 cells. (C) Left, loss of EZH2 increased BRCA1 nuclear protein level, but decreased BRCA1 cytoplasmic protein level. N and C represent nuclear and cytoplasmic protein respectively. Right, quantization of western blot. (D) Immunocytochemistry analysis shown that stable inhibition of EZH2 in A2780 cells increased BRCA1 protein expression and induced BRCA1 nuclear localization (×400).
Sodium Laureth Sulfate Standapol® Es 2, supplied by BASF, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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es-2  (JCRB Cell Bank)
90
JCRB Cell Bank es-2
Figure 1. Inhibition of EZH2 decreases BRCA1 mRNA level but increases BRCA1 protein level. (A) The mRNA level of BRCA1 was decreased in shEZH2 transfected cells compared with untransfected <t>ES2,</t> SKOV3, and A2780 cells. (B) Immunoblot assays revealed that total protein expression of BRCA1 was increased in stable transfected shEZH2-A2780 cells. (C) Left, loss of EZH2 increased BRCA1 nuclear protein level, but decreased BRCA1 cytoplasmic protein level. N and C represent nuclear and cytoplasmic protein respectively. Right, quantization of western blot. (D) Immunocytochemistry analysis shown that stable inhibition of EZH2 in A2780 cells increased BRCA1 protein expression and induced BRCA1 nuclear localization (×400).
Es 2, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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conductivity sensor (es-2  (Decagon Devices)
90
Decagon Devices conductivity sensor (es-2
Figure 1. Inhibition of EZH2 decreases BRCA1 mRNA level but increases BRCA1 protein level. (A) The mRNA level of BRCA1 was decreased in shEZH2 transfected cells compared with untransfected <t>ES2,</t> SKOV3, and A2780 cells. (B) Immunoblot assays revealed that total protein expression of BRCA1 was increased in stable transfected shEZH2-A2780 cells. (C) Left, loss of EZH2 increased BRCA1 nuclear protein level, but decreased BRCA1 cytoplasmic protein level. N and C represent nuclear and cytoplasmic protein respectively. Right, quantization of western blot. (D) Immunocytochemistry analysis shown that stable inhibition of EZH2 in A2780 cells increased BRCA1 protein expression and induced BRCA1 nuclear localization (×400).
Conductivity Sensor (Es 2, supplied by Decagon Devices, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. Inhibition of EZH2 decreases BRCA1 mRNA level but increases BRCA1 protein level. (A) The mRNA level of BRCA1 was decreased in shEZH2 transfected cells compared with untransfected ES2, SKOV3, and A2780 cells. (B) Immunoblot assays revealed that total protein expression of BRCA1 was increased in stable transfected shEZH2-A2780 cells. (C) Left, loss of EZH2 increased BRCA1 nuclear protein level, but decreased BRCA1 cytoplasmic protein level. N and C represent nuclear and cytoplasmic protein respectively. Right, quantization of western blot. (D) Immunocytochemistry analysis shown that stable inhibition of EZH2 in A2780 cells increased BRCA1 protein expression and induced BRCA1 nuclear localization (×400).

Journal: Cancer Biology & Therapy

Article Title: EZH2 participates in malignant biological behavior of epithelial ovarian cancer through regulating the expression of BRCA1

doi: 10.4161/cbt.27306

Figure Lengend Snippet: Figure 1. Inhibition of EZH2 decreases BRCA1 mRNA level but increases BRCA1 protein level. (A) The mRNA level of BRCA1 was decreased in shEZH2 transfected cells compared with untransfected ES2, SKOV3, and A2780 cells. (B) Immunoblot assays revealed that total protein expression of BRCA1 was increased in stable transfected shEZH2-A2780 cells. (C) Left, loss of EZH2 increased BRCA1 nuclear protein level, but decreased BRCA1 cytoplasmic protein level. N and C represent nuclear and cytoplasmic protein respectively. Right, quantization of western blot. (D) Immunocytochemistry analysis shown that stable inhibition of EZH2 in A2780 cells increased BRCA1 protein expression and induced BRCA1 nuclear localization (×400).

Article Snippet: Three human epithelial ovarian cancer cell lines, A2780, SKOV3, and ES2 were purchased from China Center for Type Culture Collection (CCTCC).

Techniques: Inhibition, Transfection, Western Blot, Expressing, Immunocytochemistry

Figure 2. Depletion of BRCA1 partially rescues the biological effects of EZH2 inhibition in ovarian cancer. (A and B) The efficiencies of siBRCA1s silencing in A2780 cells were detected at mRNA (A) and protein (B) levels by qRT-PCR and western blotting respectively. (C) The shEZH2-mediated BRCA1 overexpression was abolished by cotransfection of siBRCA1-2 or -3. (D) Quantization of western blot. (E and F) Cell proliferation and cell cycle analyses were detected by MTT assay and flow cytometry respectively; showing that cotransfection of siBRCA1 partly rescued the reduction in cell proliferation and depressed the elevated percentage of G2/M phase caused by shEZH2 transfection in A2780 and ES2 cells. (G) Transwell migration assays revealed that inhibition of BRCA1 inversed the suppression in cell migration in A2780 and ES2 cells with shEZH2 transfection (×200).

Journal: Cancer Biology & Therapy

Article Title: EZH2 participates in malignant biological behavior of epithelial ovarian cancer through regulating the expression of BRCA1

doi: 10.4161/cbt.27306

Figure Lengend Snippet: Figure 2. Depletion of BRCA1 partially rescues the biological effects of EZH2 inhibition in ovarian cancer. (A and B) The efficiencies of siBRCA1s silencing in A2780 cells were detected at mRNA (A) and protein (B) levels by qRT-PCR and western blotting respectively. (C) The shEZH2-mediated BRCA1 overexpression was abolished by cotransfection of siBRCA1-2 or -3. (D) Quantization of western blot. (E and F) Cell proliferation and cell cycle analyses were detected by MTT assay and flow cytometry respectively; showing that cotransfection of siBRCA1 partly rescued the reduction in cell proliferation and depressed the elevated percentage of G2/M phase caused by shEZH2 transfection in A2780 and ES2 cells. (G) Transwell migration assays revealed that inhibition of BRCA1 inversed the suppression in cell migration in A2780 and ES2 cells with shEZH2 transfection (×200).

Article Snippet: Three human epithelial ovarian cancer cell lines, A2780, SKOV3, and ES2 were purchased from China Center for Type Culture Collection (CCTCC).

Techniques: Inhibition, Quantitative RT-PCR, Western Blot, Over Expression, Cotransfection, MTT Assay, Flow Cytometry, Transfection, Migration